Tag: memories

Categories
nostalgia raves

Dotcom daftness again? Or is it real?

It’s the sort of idea that we used to talk about breathlessly in innovation centre coffee meetings with other Internet entrepreneurs, swapping stories of the latest daft idea to get a squillion dollars of funding. Not that the ideas we had weren’t daft too. My best conversations were with then Oxford graduate student Alex Straub, of the splendidly daft mondus.com (hey, I too was a believer once), who probably personally made a few million from Italian investors Seat Pagine Gialle before mondus went belly-up. Back in 1999 Alex and I would go all googly-eyed at crazy Internet ideas, the madder the better.

So here’s the idea – and it comes from a DPhil Biochemistry student at Oxford. (Hurrah for the biochemistry training – it’s so darned versatile!)

A website where you buy moments in time. Your first kiss, it’s suggested, or perhaps the moment you were offered a book deal. For $1 per minute you get to baggsy that moment and upload content which will be hosted in perpetuity, to share and share again with everyone in the world.

Thomas Whitfield apparently pitched this to Dan Wagner, himself a wily Internet entrepeneur, the guy behind the business information service M.A.I.D and then Dialog…and now the investment fund Bright Station Ventures, at a competition run by the Oxford Entrepreneurs. Instead of giving the £5,000 prize, Dan Wagner offered Whitfield and his associates access to the whole $100 million fund to develop and idea that I’m guessing they think will be the new Youtube. Wagner thinks that Designthetime (now known as miomi) captures the whole zeitgeist of the Internet.

Except…Youtube, Facebook, MySpace and all those sites on which we all frantically upload content to our hearts content…are free. What’s to stop Yahoo or Google setting up something like this, and not charging?

When the whole dotcom thing collapsed it did so largely because most of the new businesses had non-existent revenue streams, and were spending money much, much faster than they could possibly make it. The smart money flew away and settled on the few safer bets, like Google and Yahoo. So respect is due to these guys for building in a user-driven revenue stream from the beginning. But will people pay for this frivolity? It will be interesting to see.

I can’t see how this won’t be imitated. For one thing, what will I do when I discover that my special moment has been nabbed? Will I upload content for my second favourite moment? Or will I go to a rival site, one that’s quite possibly free?

I like being all sceptical, but deep down I really hope it works. It was a great feeling, the belief that a graduate student could spin a yarn and end up running a multi-million dollar business. I Googled Alex recently – he looks to be doing pretty, pretty fine.

Categories
getting published jaguar's realm Joshua Files nostalgia other books science writing

My New Editing Regime…and Memories of Subcloning


The publisher and I have agreed a deadline for Joshua bk 1 v3.0. I’m deep in the process of writing Jaguar though, and can’t let the momentum go. So I try to work on Jaguar in the morning at my desk, take a two-hour break to refresh and then it’s on with the editing, which seems to require a different skillset as far as I can tell.

Thank goodness for editors. I’ve said it before and I’ll say it again. Mine is probably going to save me from being a laughing stock, if nothing else – hopefully a lot else but you can’t predict these things.

I like to take my manuscript out for little walks. I can’t be bothered going all the way to the Bod this time around – I’m only spending 2 hours a day on it, what with the Jaguar writing taking up all my morning brain activity. So I’ve been going to Summertown.

The above photo is taken of my set-up at the Summertown Wine Cafe, a bijoux little joint on South Parade which makes the best coffee in Summertown (there are many Italian coffee machines in Summertown, but few baristas who have a clue how to use them). Sadly however, they charge a small fortune for savoury food – best to stick to cake, I’m trying to avoid blimpdom so that’s out.

Blah, blah, blah. Nothin of consequence in this entry sadly. I’m just writing something to have to test in a new way to do an RSS feed. If you read this, you’ve just participated in an experiment.

Do you feel used?

I kind of miss doing experiments. Somewhere in the back of my mind is the niggling feeling that a PROPER day’s work is what I used to pull off at the height of my keenness as a graduate student…a long day in the lab which ends with a successfully identified new DNA subclone to use in a lovely biological experiment.

‘Subcloning’ is a way of starting with a widgey little bit of DNA that is no use to anyone and two days later having bucketloads (as much as a milligram!) of the stuff that you can use to do biological experiments in tissue culture cells or even in unsuspecting fluffy creatures. (Some journals are so fussy that you can’t get published unless your results are in a live organism.)

You insert a piece of experimental DNA into a ‘vector’ of usually bacterial or yeast DNA which has the ability massively to replicate it. Then you can grow the ‘bugs’ in a 500ml culture overnight and in the morning extract enough DNA to ‘transfect’ cells which allow you to test the properties of your experimental DNA. The tricky bit is that when you try to stick your experimental DNA to the vector DNA, only a small fraction will combine to give you the subclone. The rest will just be vector DNA that sticks back to itself.

When I were a lass we used to pick at least 24 bacterial colonies in the hope that 2 or 3 would have the subclone. It could take up to a whole day, a day spent ‘doing minipreps’, as we used to call it. Sometimes you had to use radioactivity and horrible, ooky, gloopy, neurotoxic polyacrylamide gel to help identify the subclone.

(Any molecular biologists reading this, bright young things with your PCR, your DNA synthesisers and sequencing machines…it’s all very easy now, I’ll bet.)

But! Throughout most of career as a molecular biologist I noticed that although I was a good little scientist and picked my 24-48 colonies everytime I wanted to find a correctly subclone, more often than not, colony 1 (the first I picked with a sterile toothpick) actually had the subclone. i.e. I didn’t need colonies 2-24 and all the effort in ‘working them up’ was not actually essential.

Other people in my lab noticed this too. It turns out that in maths the number 1 is disproportionately represented (there’s some rule and it’s used as a way to detect fraud), well, in molecular biology this seems true too.

Don’t think we let that observation go to waste, either. Towards the end of my time in the lab, I would often just pick a colony right off, inoculate my 500ml flask and grow up the bugs without testing whether they had the subcloned DNA in them. It saved a whole day! Of course I tested a sample before I used it to transfect my tissue culture cells. Well, duh.

If you didn’t understand a thing I wrote in the last few paras, tell me. R1X did, so I have tried to rewrite it so that it makes sense.

Categories
raves

Blogs I Read

Okay, here’s a list of the blogs I regularly read:

1. Miss Snark
Yay Snarky! I worked out who she is, you know. No, really, I did. I’m not telling though. The Snark does her best work undercover.
2. Peter Cox
Well, he is my agent. 🙂
3. Richard Herring – Warming Up
The diary of a successful stand-up comedian/writer. Richard and I went to the same college, although we never spoke, so far as I know. He spent little time there. He told me, years ago, that he hated the college. I corresponded with him by email briefly during the nineties. He makes himself write something everyday; admirable pursuit.
4. MaryD
MaryD is the mother of my best friend-when-we-were-kids. I remember the cakes she used to make to this day. She did lovely vanilla Victoria sponges iced with orange and lemon buttercream. Then there was this unbelievable chocolate hazelnut cake filled with whipped cream, the first baking of which has a place of honour in my memories. I’m willing to forget a whole year of biochemistry to make the necessary space for the details in my crowded memory.
MaryD now lives in a village in Co. Galway and uses her brilliance as a journalist to paint a fascinating view of aspects of life in a rural Ireland.

Right, now for blogs I’d like to read.

Categories
switzerland

Bizarro Coincidence

As I lay in hospital, as coincidence would have it, the patient who joined me in the small, immaculately clean and tidy Swiss hospital ward, was from Mexico.

An Olympic standard beach volleyball player, poor girl, she’d broken her wrist. Not skiing, either, but falling off a bar stool or something.

So, out of all the Mexicans in the valley, we ended up in adjacent hospital beds. Klutzes or what?

We started to chat. The young woman’s mind was, not surprisingly, turning to thoughts of a post-volleyball career. I asked her what she’d studied and where. Personnel administration, at the UNAM. Well then, I offered, maybe you’ve read my grandfather’s book. He’s Agustin Reyes Ponce.

And that was the strangest part of all. That two crumbly-boned Mexicans should meet at the base of a wintry ski slope, I buy. That one should be in awe of the other for being an Olympic athlete…okay. That the other should be silenced in respectful memory of a deceased guru of the Mexican business schools, was taking it all too far.

How big is this world, anyway?